Interference Microspectroscopy

A novel ATR FT-IR microspectroscopy technique for surface contamination analysis without interference of the substrate.

Surface contaminants, such as powder and thin film on various solid surfaces, were analyzed by ATR FT-IR microspectroscopy. An ATR accessory consisting of a miniature-Ge IRE with contact area smaller than 50 microm, in diameter was fabricated and employed for a non-destructive characterization. The IRE was pre-aligned and fixed onto a 15x Schwarzschild-Cassegrain infrared objective. Easy maneuv...

Vibrational Microspectroscopy for Cancer Screening

Vibrational spectroscopy analyses vibrations within a molecule and can be used to characterise a molecular structure. Raman spectroscopy is one of the vibrational spectroscopic techniques, in which incident radiation is used to induce vibrations in the molecules of a sample, and the scattered radiation may be used to characterise the sample in a rapid and non-destructive manner. Infrared (IR) s...

Infrared microspectroscopy in biological research.

FTIR Microspectroscopy of Polymeric Systems

Fourier transform infrared (FTIR) spectroscopy is a mature analytical technique employed to examine polymeric materials. With the coupling of an infrared interferometer to a microscope equipped with specialized detectors, FTIR spectroscopy has been employed widely to examine microscopic areas in polymers for the last twenty years. Following the emergence of instrumentation techniques that emplo...

Raman Microspectroscopy of LiDNA Fibres

The conformation of low-salt LiDNA in fibrous form has been studied by Raman microspectroscopy. The recorded spectrum of a fibre soaked in ethanol is very similar to that of a fibre at low relative humidity. From x-ray experiments it is known that in the latter case the DNA adopts the C conformation. Therefore, it can be concluded that this is also the case in a low-salt LiDNA fibre soaked in e...